Every living cells contains DNA, RNA and protiens. closely related organisms generally have degree of agreement in the molecular structure of these substances,Heterogenous nuclear ribonucleoprotiens are spliceosomal macromolecular assemblages and thus actively participate in pre-mRNA metabloism.
conserved sequnces. such as mitochondrial DNA are expected to accumalate mutations over time and assuming a constant rate of mutation provide molecular interaction features.
Molecular characterization of human hnRNP A3 showed that while the recombinant hnRNP A3 with its 296 amino acids migrates as excpted as 32 kDA protien on SDS-PAGE analysis, it is recoginzed by the patient's sera as a 50 kda highly related ye unknownv crossreactive protien.
The sequence comparision. however may be insufficient for deduction of its functional role patients diagnozed with the suspected disease. therefore we followed the suggestion of ponting CS and Russel RRSurprisingly, neither the 50 kda nor the 32kda protien was dected in the suspected disease.